Recombinant CD163 anticorps

N° du produit ABIN7487685

L’anticorps Souris Monoclonal anti-CD163 a été validé pour FACS, ELISA et IF. Il convient pour détecter CD163 dans des échantillons de Porc.

Aperçu rapide pour Recombinant CD163 anticorps (ABIN7487685)

Antigène: CD163

Type d'anticorp: Recombinant Antibody

Reactivité: Porc

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD163 est non-conjugé

Application: Flow Cytometry (FACS), ELISA, Immunofluorescence (IF)

Clone: 2A10

Détail du produit anti-CD163 anticorps

Fonction: Anti-CD163 [2A10 (2A10/11)], Mouse IgG1, lambda

Specificité: This antibody recognizes and binds porcine CD163.

Attributs du produit:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogène: The original antibody was generated by immunizing BALB/c mice with porcine alveolar macrophages.

Isotype: IgG1 lambda

Information d'application

Indications d'application: This antibody is recommended to be used as a tool in the study of porcine macrophage maturation and differentiation and for determining their heterogeneity both in normal and pathological conditions. This antibody can recognize porcine CD163 in a western blot assay and an immunoprecipitation assay (PMID: 9151393). 2A10 successfully binds PAM cells in a flow cytometric analysis (PMID:23785195). Porcine CD163 has also been analyzed as potential antigen targeting receptors using mouse IgG as surrogate antigens. MAbs to CD163 induced in vitro PBMC proliferation at concentrations 50-80 fold lower than the control mAb when using, as responder cells, cells from pigs immunized with mouse serum IgGs (PMID:21557980). It is also reported that porcine mAb 2A10 antigen is homologous to human CD163 and related to macrophage differentiation (PMID: 10227997). This antibody was also used to study the role of CD163 in the process of infection of porcine monocytes/macrophages by African swine fever virus infection (PMID: 14648288). This mAb was used in a sandwich enzyme-linked immunoassay (ELISA) to measure levels of sCD163 in porcine sera and biological fluids (Pérez et al, 2008).

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur CD163

Antigène: CD163

Autre désignation: CD163

Sujet: M130, sCD163, Scavenger receptor cysteine-rich type 1 protein M130

UniProt: Q2VL90